Vivacon 500-PCR Grade

Vivacon 500-PCR Grade


Cat. No. : 2SRVS-VN01H21ETO
Brand : : Sartorius Lab Instruments




For up to 500 µl
Vivacon® 500 are centrifugal units for fast DNA concentration and buffer exchange of 500 µl samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.

Vivacon® 500-PCR Grade units are treated with ethylene oxide (ETO) in a validated process to deactivate all traces of DNA that might interfere with subsequent amplification procedures.

Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.

  • Concentration of up to 0.5 ml genomic DNA or peptide samples
  • Low binding material
  • Reverse spin possibility
  • MWCO printed on device
  • Complete and highly reproducible concentrate recovery
  • Selected cut offs available sterile and DNA free* for e.g. forensic applications
  • * qPCR assay over 55 cycles with a verified limit of detection < 2 pg

Performance Characteristics for DNA

Start volume 0.5 ml, sample concentration 50 ng/ml.

  Sample size (bp) Time to concentrate up
to 30x [min.] at 20°C
Concentrate
recovery %
g-force (xg)
2,000 MWCO 10 60 min. 93 % 7,500
10,000 MWCO 30 25 min. 94 % 7,500
30,000 MWCO 50 18 min. 88 % 5,000
50,000 MWCO 300 18 min. 91 % 5,000
100,000 MWCO 600 10 min. 87 % 3,000
125,000 MWCO 650 12 min. 85 % 2,000
125,000 MWCO 900 9 min. 94 % 3,000

Performance Characteristics for Proteins

Start volume 0.5 ml, sample and concentration of proteins as specified in table.

  Sample Time to concentrate up
to 30x [min.] at 20°C
Concentrate
recovery %
g-force (xg)
2,000 MWCO 0.25 mg/ml
cytochrome c
30 min. 95 % 14,000
10,000 MWCO 0.25 mg/ml
cytochrome c
15 min. 92 % 14,000
30,000 MWCO 1.0 mg/ml BSA 10 min. 95 % 14,000
50,000 MWCO 1.0 mg/ml BSA 10 min. 92 % 14,000
100,000 MWCO 1.0 mg/ml
bovine IgG
11 min. 90 % 8,000
125,000 MWCO 1.0 mg/ml
bovine IgG
10 min. 81 % 8,000

 

Product properties  
MWCO 30,000 Daltons
Volume Range 0.1 – 0.5 mL
Primary Product Type Ultrafiltration Devices
PCR Grade Yes
Membrane material Hydrosart®
Membrane Hydrosart<sup>®</sup>
Technical attributes  
Electrical / battery item false
Concentrator Body/ Sleeve Polycarbonate (PC)
Dead stop volume 5 µL
Certification None
Filtrate Vessel Polypropylene (PP)
Application Protein Concentration` DNA Concentration
Area of application Buffer Exchange` Forensic Sample Prep` Protein/DNA Concentration
Device typeCentrifugal Ultrafiltration Devices Forensic
Device type Centrifugal Ultrafiltration Devices
Sample Type DNA

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Vivacon® 500 and Vivacon® 2 — For safe DNA and protein concentration and rebuffering from 500 μl to 2 ml starting volumes. Click Here

Vivacon® 500 — For in vitro use only Click Here

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